GFP His Tag protein, Aequorea victoria Size:50μg Carefully remove the supernatant

Carefully remove the supernatant

and then it was shaken at 4 ℃ for 20-25min or left to digest for 40-50min (observe the digestion at any time during the digestion)

determine the hole in the 450 - nm wavelength

4) Return to the standard curve to calculate to calculate when diluted multiples (x 40)

GFP His Tag protein, Aequorea victoria Size:50μg Carefully remove the supernatantProduct Specification Species Aequorea victoria Antigen GFP Synonyms GFP Protein Accession AAB65663 Amino Acid Sequence Ser2 Lys238, with N terminal 8*His HHHHHHHHSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK Expression System E. coli Molecular Weight 27. 8kDa